LOCUS       40924_26764        3882 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKITE101 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Toda H, Koyanagi T, Enomoto T, Itoh N.
  TITLE     Characterization of two cryptic plasmids from Kocuria palustris 
            IPUFS-1 and construction of novel Escherichia coli-Kocuria shuttle 
            vector for biocatalysis
  JOURNAL   J. Biosci. Bioeng. 124 (3), 255-262 (2017)
  PUBMED    28495560
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Itoh N, Toda H, Koyanagi T, Enomoto T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural 
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 3882)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci. 
            Bioeng."; date: "2017"; volume: "124"; issue: "3"; pages: "255-262"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural University, 
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http"; pages: 
            "//www.pu-toyama.ac.jp/BR/itoh"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2270..3876
                     /plasmid="pKPAL1"
                     /strain="IPUFS-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:71999"
                     /organism="Kocuria palustris"
     protein_bind    39..60
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        75..105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    162..218
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(222..238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(460..1272)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1654..2242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2402..3277
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /protein_id="BAX51295.1"
                     /translation="MTTAECVQRWDQMWLPLWPLASDDLHAGVYRQARGEAMHRRYIET
                     NPRALSNLLVVDIDHEDAALRTMWNRQAWWPNAVVENPSNGHAHAVWALNEPITRTEYA
                     RRKPLAFAAAVTEGLRRSVDGDTGYSGLLTKNPAHDDWDALWCNSDRLYDLDELAEHLT
                     EGGYMPPVSWKRSKRRNSVGLGRNCSIFETARTWAYREIRNHWFDSAGLHDAISAHVHE
                     LNAEFPEPLPASEARAIAASIHRWITTKSRMWADGPAVYEATFSTIQAARGRKRVERTK
                     ALLEGWQPNG"
     gene            2402..3277
                     /gene="repA"
                     /label=repA
     CDS             3270..3578
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label=repB
                     /protein_id="BAX51296.1"
                     /translation="MGDRYDRKGSSISEMSRGTGLSPATIKRWTSRSRDEWLQQKADER
                     EAIRAFHDDEGHSWPQTAKHFRLDVSTVKRRAYRAREERKQEIAEQLQPPLPFPTNS"
     gene            3270..3578
                     /gene="repB"
                     /label=repB
ORIGIN
        1 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       61 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      121 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag ctcggtaccc
      181 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
      241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      361 agcctgaatg gcgaatgcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat
      421 gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
      481 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      541 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      601 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      661 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      721 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      781 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      841 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      901 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      961 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     1021 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     1081 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     1141 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     1201 atataaatca gcatccatgt tggaatttaa tcgcggcttc gagcaagacg tttcccgttg
     1261 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     1321 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     1381 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     1441 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     1501 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
     1561 gtatgagtca gcaacacctt cttcacgagg cagacctctc gacggatcgt tccactgagc
     1621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1801 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2041 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttagatctt gcggctgaac
     2281 gaacacgggc accccagggg caaagcggac tgcgctgcgc gcgggtgggt ggatgcccga
     2341 aacatctgac ttggctaaga gccactttgc accgggtcat gtttgtggga gagtcgcaca
     2401 catgacgacg gcggagtgcg tgcagcggtg ggatcagatg tggttgccgc tgtggccgtt
     2461 ggcctccgac gacctgcacg cgggtgtgta ccggcaggcg cgcggtgagg cgatgcatcg
     2521 tcgctacatc gagacgaacc cgcgtgcgct gagcaacctg ctcgtggtgg acatcgacca
     2581 cgaggacgcc gcgctgcgca cgatgtggaa ccgacaggcc tggtggccca acgcggtcgt
     2641 ggagaacccg tccaacggcc acgcgcacgc cgtctgggcg ctcaacgaac cgatcacccg
     2701 cacggagtac gcccgtcgca agccgctggc gttcgcagca gctgtgaccg aggggctgcg
     2761 ccgctccgtg gacggcgaca cgggctactc cgggctgctg accaagaacc ccgcacacga
     2821 cgactgggac gcgttgtggt gcaacagcga ccggctctac gacctcgacg agctcgctga
     2881 gcacctcacc gagggcgggt acatgcctcc ggtgtcctgg aagcgctcca agcgccgcaa
     2941 cagcgtcggg ctgggccgca actgctcgat cttcgagacc gcacgcacct gggcctaccg
     3001 cgagatccgc aaccactggt tcgacagtgc cggcctccac gacgcgatca gcgcacacgt
     3061 gcacgaactc aatgccgagt tccccgaacc actgccggca tcagaagccc gagcgatcgc
     3121 ggccagcatc caccgctgga tcaccaccaa gtcccgcatg tgggccgacg gccccgccgt
     3181 ctacgaagcc acgttctcca ccatccaagc cgcacgcggg cgcaagcgcg tggaacgcac
     3241 caaggcactc ttggaggggt ggcagcccaa tgggtgatcg gtacgaccgc aaggggagtt
     3301 ccatctcgga gatgtcgcga ggaactggtc tgagcccagc cacgatcaag cgatggacct
     3361 cgcgtagtcg ggatgagtgg cttcagcaga aagccgacga gcgtgaggcc atccgtgcgt
     3421 tccacgacga tgaggggcac tcctggccgc agacggccaa gcacttccgg ctcgacgtct
     3481 cgacggtgaa gcgccgcgcg tatcgagcgc gtgaggagcg caaacaggag atcgccgagc
     3541 agttgcagcc tccgctgccc ttccccacca actcctgaac catcagctat caaaaacgaa
     3601 attcgccccc gggactccga cggagccccg ggggcgaatt tccggtatat ggtataatgt
     3661 ccttagcgat cattccaagc agtgccatgc ctgggattcc ggtcgcccga ggagatggtc
     3721 catatccgta gtgctctgta tatacattac gttatcagga ctgcgacatc agagcaaccg
     3781 tggactccca cgactgcccc gtcgcgacgg cccaagcggg ccgcccctac cgacctcctc
     3841 tcctcggacc ttcgtcgcgc cgaggagagg aggaacccat gg
//
